Application
FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. FOXA1 antibody dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1 antibody at 1:1000 dilution.Application
FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. FOXA1 antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.Application
FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 μg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 μg of FOXA1 antibody 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | FOXA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FOXA1 (forkhead box A1)This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HNF3A antibody MGC33105 antibody TCF3A antibody FOXA1 antibody HNF-3-alpha antibody HNF-3A antibody hepatocyte nuclear factor 3-alpha antibody "hepatocyte nuclear factor 3 alpha antibody" transcription factor 3A antibody forkhead box protein A1 a |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Nucleus |
Application
FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. FOXA1 antibody dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1 antibody at 1:1000 dilution.
Application
FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. FOXA1 antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
Application
FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 μg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 μg of FOXA1 antibody 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
