Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) AntibodyApplication
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (Phospho-Ser256) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR (Phospho-Ser256) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256) Antibody .| Product Name | FOXO1 (phospho Ser256) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FOXO1 (phospho Ser256)Transcription factor. |
| Immunogen | Peptide sequence around phosphorylation site of serine 256 (A-A-S(p)-M-D) derived from Human FKHR. |
| Modification | p-Ser256 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FKH1 FKHR FOXO1A FOXO1 forkhead Drosophila homolog of in rhabdomyosarcoma forkhead box protein O1 forkhead box protein O1A forkhead box O1 |
|---|---|
| Target | FOXO1 (phospho Ser256) |
| Molecular Weight(MW) | 78-82 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) Antibody
Application
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (Phospho-Ser256) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR (Phospho-Ser256) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
