Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197)Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (Phospho-Ser197) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197) Antibody .| Product Name | FOXO4 (phospho Ser197) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AFX (Phospho Ser197)Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. |
| Immunogen | Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX. |
| Modification | p-Ser197 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AFX AFX1 MGC120490 MLLT7 FOXO4 forkhead box protein O4 fork head domain transcription factor AFX1 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog Drosophila) translocated to 7 forkhead box O4 |
|---|---|
| Target | AFX (Phospho Ser197) |
| Molecular Weight(MW) | 65 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197)
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (Phospho-Ser197) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
