| Product Name | FOXO4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FOXO4The forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX and FKHRL1) have sequence similarity to the nematode homolog Daf 16, recently shown to mediate signaling via a pathway involving IGFR1, P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site, FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253. |
| Immunogen | Synthetic peptide, corresponding to C-term amino acids 451-466 with N-terminally added lysine, of Human AFX / FOXO4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/1000. This dilution is determined by using paraformaldehyde fixed AFX transfected COS-7 cells. WB: Use at a dilution of 1/2000. This dilution is determined using human AFX expressed in transfected COS-7 cell extracts. Predicted molecular weight: 50 kDa. Staining of AFX is specifically inhibited by the AFX immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AFX1 4303 300033 AFX FOXO4 MGC120490 MLLT7 P98177 |
|---|---|
| Target | FOXO4 |
| Positive Control | AFX transfected COS-7 cells |
|---|---|
| Application Notes | IF: Use at a dilution of 1/1000. This dilution is determined by using paraformaldehyde fixed AFX transfected COS-7 cells. WB: Use at a dilution of 1/2000. This dilution is determined using human AFX expressed in transfected COS-7 cell extracts. Predicted molecular weight: 50 kDa. Staining of AFX is specifically inhibited by the AFX immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
