Application
Western blot analysis of extracts from 293 and THP1 cells using AFX(Ab-197) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AFX Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 and THP1 cells using AFX Antibody .| Product Name | FOXO4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AFXTranscription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. |
| Immunogen | Peptide sequence around aa. 195~199 (A-A-S-M-D) derived from Human AFX. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AFX AFX1 MGC120490 MLLT7 FOXO4 forkhead box protein O4 fork head domain transcription factor AFX1 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog Drosophila) translocated to 7 forkhead box O4 |
|---|---|
| Target | AFX |
| Molecular Weight(MW) | 65 (note) |
Application
Western blot analysis of extracts from 293 and THP1 cells using AFX(Ab-197) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AFX Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 and THP1 cells using AFX Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
