Application
Immunocytochemistry/Immunofluorescence: FOXP3 Antibody [ - Sample: Healthy thymus from a C57BL/6 mouseApplication
Western Blot: FOXP3 Antibody [ - Detection of human FOXP3 using Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vectorApplication
Immunocytochemistry/Immunofluorescence: FOXP3 Antibody [ - Incubated only with CD4 and no Foxp3| Product Name | FOXP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FOXP3FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells. |
| Immunogen | A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| For IHC-Fr: Use at a dilution of 1:50. For WB: Use at a dilution of 1:1,000-1:5,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | 50943 PIDX Q9BZS1 AIID DIETER JM2 222100 XPID IPEX FOXP3 |
|---|---|
| Target | FOXP3 |
| Tissue Specificity | This antibody is specific for human FOXP3. |
Application
Immunocytochemistry/Immunofluorescence: FOXP3 Antibody [ - Sample: Healthy thymus from a C57BL/6 mouse
Application
Western Blot: FOXP3 Antibody [ - Detection of human FOXP3 using Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector
Application
Immunocytochemistry/Immunofluorescence: FOXP3 Antibody [ - Incubated only with CD4 and no Foxp3| Application Notes | For IHC-Fr: Use at a dilution of 1:50. For WB: Use at a dilution of 1:1,000-1:5,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Tris-citrate/phosphate, pH 7-8, containing sodium azide |
