Application
Western blot analysis of FUCA1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). FUCA1 (arrow) was detected using the purified Pab.Application
FUCA1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
FUCA1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUCA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | FUCA1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FUCA1 (Internal)The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2. |
| Immunogen | KLH conjugated synthetic peptide between 272~302 amino acids from the Center region of human FUCA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Alpha-L-fucosidase 1 Tissue alphaLfucosidase AlphaLfucosidase1 Alpha-L-fucosidase I AlphaLfucosidase I Tissue alpha-L-fucosidase FUCA1 Nbla10230 AlphaLfucosidase 1 AlphaLfucoside fucohydrolase Alpha-L-fucoside fucohydrolase Alpha L fucosidase 1 |
|---|---|
| Target | FUCA1 |
| Molecular Weight(MW) | 53.689 kDa (note) |
Application
Western blot analysis of FUCA1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). FUCA1 (arrow) was detected using the purified Pab.
Application
FUCA1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
FUCA1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUCA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
