Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.Application
Fumarate hydratase antibody detects Fumarate hydratase protein by western blot analysis. A.50 µg rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Fumarate hydratase antibody detects FH protein by western blot analysis. A. 50 µg mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Fumarate hydratase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fumarate hydratase (fumarate hydratase)The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Pig, Chicken, Sheep, Chimpanzee, BovineZebrafish (90%), Pig (98%), Chicken (91%), Sheep (99%), Chimpanzee (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HLRCC antibody LRCC antibody MCL antibody MCUL1 antibody FH antibody fumarase antibody fumarate hydratase mitochondrial antibody fumarate hydratase antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
| Cellular Localization | Mitochondrion , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.
Application
Fumarate hydratase antibody detects Fumarate hydratase protein by western blot analysis. A.50 µg rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Fumarate hydratase antibody detects FH protein by western blot analysis. A. 50 µg mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , HeLa , HepG2 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
