Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using G6PD antibody at 1:300 dilution.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with G6PD antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: HeLa S3 7.5% SDS PAGE diluted at 1:500| Product Name | G6PD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G6PD (glucose-6-phosphate dehydrogenase)This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rabbit, Sheep, Rhesus Monkey, BovineXenopus laevis (82%), Rabbit (95%), Sheep (92%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | G6PD1 antibody G6PD antibody "glucose-6-phosphate dehydrogenase G6PD antibody" glucose-6-phosphate 1-dehydrogenase antibody glucose-6-phosphate dehydrogenase antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using G6PD antibody at 1:300 dilution.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with G6PD antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: HeLa S3 7.5% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
