Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody diluted at 1:2000.Application
G9a antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 μg of G9a antibody 5% SDS-PAGE The immunoprecipitated G9a protein was detected by G9a antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
G9a antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. G9a antibody diluted at 1:250.Application
G9a antibody detects G9a protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G9a protein stained by G9a antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-G9a antibody. The precipitated DNA was detected by PCR with primer set targeting to FXN.Application
G9a antibody detects G9a protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract | D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE G9a antibody dilution: 1:1000Application
G9a antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody diluted at 1:3000.| Product Name | G9a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G9a (euchromatic histone-lysine N-methyltransferase 2)A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human G9a. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (99%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GAT8 G9A C6orf30 KMT1C BAT8 euchromatic histone-lysine N-methyltransferase 2 NG36 EHMT2 |
|---|---|
| Target | G9a |
| Molecular Weight(MW) | 132 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody diluted at 1:2000.
Application
G9a antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 μg of G9a antibody 5% SDS-PAGE The immunoprecipitated G9a protein was detected by G9a antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
G9a antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. G9a antibody diluted at 1:250.
Application
G9a antibody detects G9a protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G9a protein stained by G9a antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-G9a antibody. The precipitated DNA was detected by PCR with primer set targeting to FXN.
Application
G9a antibody detects G9a protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract | D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE G9a antibody dilution: 1:1000
Application
G9a antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody diluted at 1:3000.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
