Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 5% SDS PAGE diluted at 1:1000| Product Name | G9a antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G9a (euchromatic histone lysine N-methyltransferase 2)Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also methylates histone H1. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of mouse G9a. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Pig, BovineHuman (98%), Rat (100%), Pig (98%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | euchromatic histone-lysine N-methyltransferase 2 antibody H3-K9-HMTase 3 antibody "histone-lysine N-methyltransferase H3 lysine-9 specific 3 antibody" HLA-B associated transcript 8 antibody protein G9a antibody histone-lysine N-methyltransferase EHMT |
|---|---|
| Molecular Weight(MW) | 138 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 5% SDS PAGE diluted at 1:1000| Positive Control | NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
