Application
Sample (30 µg of whole cell lysate) A: Neuro2A 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using GABA A Receptor alpha 5 antibody at 1:500 dilution.| Product Name | GABA A Receptor alpha 5 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GABA A Receptor alpha 5 (gamma-aminobutyric acid (GABA) A receptor, alpha 5)GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GABA A Receptor alpha 5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC138184 antibody GABRA5 antibody GABA(A) receptor subunit alpha-5 antibody gamma-aminobutyric acid receptor subunit alpha-5 antibody "gamma-aminobutyric acid (GABA) A receptor alpha 5 antibody" |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (3) |
| Cellular Localization | Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein , Cell membrane; Multi-pass membrane protein |
Application
Sample (30 µg of whole cell lysate) A: Neuro2A 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using GABA A Receptor alpha 5 antibody at 1:500 dilution.| Positive Control | NT2D1 , PC-3 , U87-MG , SK-N-SH , Neuro2A |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
