Application
Western Blot: GABA A Receptor delta Antibody [ - Affinity purified rabbit polyclonal antibody.| Product Name | GABA A Receptor delta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GABA A receptor deltaGamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). More recently there have been a number of studies demonstrating that the a-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002). |
| Immunogen | Fusion protein from the N-terminus of the rat GABA-A receptor delta subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIPWB |
| We recommend the following starting dilutions: immunoprecipitation 10 ul per 50 ug lysate, Western Blot 1:1,000, Immunohistochemistry-Frozen 1:250. Optimal concentration should be determined experimentally by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | gamma-aminobutyric acid (GABA) A receptor gammaaminobutyric acid (GABA) A receptor GABAalpha receptor beta2 subunit GABRB3 MGC9051 GABAA receptor beta3 subunit GABA-alpha receptor beta-2 subunit GABA(A) receptor beta3 subunit 256329689 GABA(A) rec |
|---|---|
| Target | GABA A receptor delta |
| Tissue Specificity | NB300-200 reacts with the 52 kDa delta subunit of rat brain GABA-A. There is no known reactivity with other GABA subunits. There is no homology with other GABA subunits. |
| Cellular Localization | Multi-pass membrane protein |
Application
Western Blot: GABA A Receptor delta Antibody [ - Affinity purified rabbit polyclonal antibody.| Application Notes | We recommend the following starting dilutions: immunoprecipitation 10 ul per 50 ug lysate, Western Blot 1:1,000, Immunohistochemistry-Frozen 1:250. Optimal concentration should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | 10mM HEPES (pH 7.5), 150mM NaCl, 100µg/ml BSA and 50% glycerol. |
