Application
A. Western blot analysis on (A) fetal brain (B) mouse brain and (C) rat brain lysates using anti-GAD2 RabMAb (cat. # ) dilution 1:1000.| Product Name | GAD65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [EPR2553Y] to GAD65Glutamate decarboxylase (GAD) catalyzes the production of gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter. GAD is represented by two isoforms, GAD1 and GAD2, which are the products of two different genes and differ substantially only at their N-terminal regions. In neurons and beta cells, GAD is concentrated around synaptic vesicles and synaptic-like microvesicles, respectively, as well as in the area of the Golgi complex. GAD2 lies primarily in axon terminals and only about half of GAD2 exists as active holoenzyme . The beta cell-specific marker GAD2 can be quantified in pancreatic cell extracts and in serum . GAD2 is a major target for autoantibodies and autoreactive T cells in patients with insulin-dependent diabetes mellitus. Autoantibodies to GAD are also found in patients with stiff man syndrome or polyendocrine autoimmunity . |
| Immunogen | A synthetic peptide corresponding to residues in human GAD2 was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR2553Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500 - 2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GAD2 MGC161607 GAD65 MGC161605 |
|---|---|
| Target | GAD65 |
| Molecular Weight(MW) | 65 (note) |
Application
A. Western blot analysis on (A) fetal brain (B) mouse brain and (C) rat brain lysates using anti-GAD2 RabMAb (cat. # ) dilution 1:1000.| Application Notes | For WB: Use at a dilution of 1:500 - 2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
