Application
GAD67 antibody detects GAD67 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD67 antibody diluted at 1:500.Application
GAD67 antibody detects GAD67 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 protein stained by GAD67 antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GAD67 antibody detects GAD67 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells 7.5% SDS-PAGE GAD67 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GAD67 antibody detects GAD67 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: GAD67 protein stained by GAD67 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. GAD67 antibody dilution: 1:500.Application
GAD67 antibody detects GAD67 protein by western blot analysis. A. 30 µg SK-N-SH whole cell lysate/extract B. 30 µg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE GAD67 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GAD67 antibody detects GAD67 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GAD67 protein stained by GAD67 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .| Product Name | GAD67 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAD67 (glutamate decarboxylase 1 (brain, 67kDa))This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Cat, Pig, Chicken, Chimpanzee, BovineDog (99%), Cat (99%), Pig (99%), Chicken (90%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ45882 antibody GAD antibody SCP antibody GAD1 antibody GAD-67 antibody glutamate decarboxylase 67 kDa isoform antibody glutamate decarboxylase 1 antibody 67 kDa glutamic acid decarboxylase antibody glutamate decarboxylase 1 (brain 67kDa) antibo |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (2) |
Application
GAD67 antibody detects GAD67 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD67 antibody diluted at 1:500.
Application
GAD67 antibody detects GAD67 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 protein stained by GAD67 antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GAD67 antibody detects GAD67 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells 7.5% SDS-PAGE GAD67 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GAD67 antibody detects GAD67 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: GAD67 protein stained by GAD67 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. GAD67 antibody dilution: 1:500.
Application
GAD67 antibody detects GAD67 protein by western blot analysis. A. 30 µg SK-N-SH whole cell lysate/extract B. 30 µg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE GAD67 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GAD67 antibody detects GAD67 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GAD67 protein stained by GAD67 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .| Positive Control | SK-N-SH , SK-N-AS , mouse brain , rat brain , human GAD1-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
