Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE Galactosidase alpha antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 μg of Galactosidase alpha antibody [N1C2] . Western blot analysis was performed using Galactosidase alpha antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Galactosidase alpha antibody [N1C2] detects Galactosidase alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Galactosidase alpha antibody detects Galactosidase alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha antibody at 1:100 dilution.| Product Name | Galactosidase alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Galactosidase alpha (galactosidase, alpha)This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Galactosidase alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | PigPig (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GALA antibody GLA antibody agalsidase alfa antibody alpha-gal A antibody melibiase antibody alpha-D-galactoside galactohydrolase 1 antibody alpha-galactosidase A antibody alpha-D-galactosidase A antibody "galactosidase alpha antibody" |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
| Cellular Localization | Lysosome |
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE Galactosidase alpha antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 μg of Galactosidase alpha antibody [N1C2] . Western blot analysis was performed using Galactosidase alpha antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Galactosidase alpha antibody [N1C2] detects Galactosidase alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Galactosidase alpha antibody detects Galactosidase alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha antibody at 1:100 dilution.| Positive Control | 293T , HeLa , Rat Lung |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
