Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:500| Product Name | GALE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GALE (UDP-galactose-4-epimerase)This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GALE. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Rhesus Monkey, BovineMouse (93%), Rat (93%), Zebrafish (82%), Rhesus Monkey (98%), Bovine (97%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ95174 antibody FLJ97302 antibody SDR1E1 antibody GALE antibody galactowaldenase antibody UDP galactose-4'-epimerase antibody "galactose-4-epimerase UDP- antibody" "short chain dehydrogenase/reductase family 1E member 1 antibody" UDP-galactose |
|---|---|
| Target | GALE |
| Molecular Weight(MW) | 38 kDa (note) (4) |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:500| Positive Control | A431 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
