Application
Western Blot: Galectin 7 Antibody - Detection of Mouse Galectin 7 on HeLa whole cell lysate using .| Product Name | Galectin 7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Galectin 7The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control. |
| Immunogen | Immunogen was a synthetic peptide, which represented a portion of human Lectin, Galactoside-binding 7 encoded within exon 4 (LocusLink ID 3963). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a concentration of 10 - 20 μg/mg of lysate. WB: Use at a dilution of 1/10000 - 1/40000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Galectin 7 PIG1 3963 P47929 GAL7 TP53I1 LGALS7 Galectin7 600615 |
|---|---|
| Target | Galectin 7 |
Application
Western Blot: Galectin 7 Antibody - Detection of Mouse Galectin 7 on HeLa whole cell lysate using .| Positive Control | Modified RIPA extract from the epidermis of FVB wild-type or galectin 7 transgenic mice |
|---|---|
| Application Notes | IP: Use at a concentration of 10 - 20 μg/mg of lysate. WB: Use at a dilution of 1/10000 - 1/40000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Tris-citrate/phosphate. pH 7-8 |
