Application
gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using gamma Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
gamma Tubulin antibody detects gamma Tubulin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. gamma Tubulin antibody dilution: 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using gamma Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. NIH3T3 whole cell extracts (30 µg) and rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of gamma Tubulin protein from Jurkat whole cell extracts using 5 μg of gamma Tubulin antibody . Western blot analysis was performed using gamma Tubulin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | gamma Tubulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to gamma Tubulin (tubulin, gamma 1)This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human gamma Tubulin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRatZebra Fish |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Rhesus Monkey, Chimpanzee, BovineZebrafish (98%), Xenopus laevis (99%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GCP-1 antibody, TUBG antibody, TUBGCP1 antibody, TUBG1 antibody, gamma-1-tubulin antibody, "tubulin, gamma polypeptide antibody", gamma-tubulin complex component 1 antibody, tubulin gamma-1 chain antibody, "tubulin, gamma 1 antibody" |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (2) |
| Cellular Localization | Centrosome |
Application
gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using gamma Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
gamma Tubulin antibody detects gamma Tubulin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. gamma Tubulin antibody dilution: 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using gamma Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. NIH3T3 whole cell extracts (30 µg) and rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of gamma Tubulin protein from Jurkat whole cell extracts using 5 μg of gamma Tubulin antibody . Western blot analysis was performed using gamma Tubulin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
