Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATM antibody [N3C3] diluted at 1:500.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GATM antibody [N3C3] diluted at 1:500.| Product Name | GATM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GATM (glycine amidinotransferase (L-arginine:glycine amidinotransferase))This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GATM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, BovineRat (97%), Zebrafish (87%), Xenopus laevis (91%), Dog (95%), Pig (94%), Chicken (91%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AGAT antibody, AT antibody, GATM antibody, transamidinase antibody, L-arginine:glycine amidinotransferase antibody, "glycine amidinotransferase, mitochondrial antibody", glycine amidinotransferase (L-arginine:glycine amidinotransferase) antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (3) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side (Potential) , Cytoplasm |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATM antibody [N3C3] diluted at 1:500.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GATM antibody [N3C3] diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
