Application
GCDH antibody detects GCDH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GCDH antibody dilution: 1:500.Application
GCDH antibody detects GCDH protein at Mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: GCDH protein stained by GCDH antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE GCD antibody diluted at 1:1000| Product Name | GCDH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GCDH (glutaryl-CoA dehydrogenase)The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GCDH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, BovineRat (88%), Xenopus laevis (83%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACAD5 antibody, GCD antibody, GCDH antibody, "glutaryl-CoA dehydrogenase, mitochondrial antibody", glutaryl-Coenzyme A dehydrogenase antibody, glutaryl-CoA dehydrogenase antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
GCDH antibody detects GCDH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GCDH antibody dilution: 1:500.
Application
GCDH antibody detects GCDH protein at Mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: GCDH protein stained by GCDH antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE GCD antibody diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
