Application
GCP4 antibody detects GCP4 protein at cytoplasm and centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GCP4 protein stained by GCP4 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
GCP4 antibody detects TUBGCP4 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract 10 % SDS-PAGE GCP4 antibody dilution: 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using GCP4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.| Product Name | GCP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GCP4 (tubulin, gamma complex associated protein 4)Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GCP4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rabbit, Chimpanzee, BovineZebrafish (90%), Xenopus laevis (90%), Rabbit (97%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 76P antibody, FLJ14797 antibody, GCP4 antibody, TUBGCP4 antibody, gamma-ring complex protein 76 kDa antibody, gamma-tubulin complex component 4 antibody, gamma tubulin ring complex protein (76p gene) antibody, "tubulin, gamma complex associated protein 4 a |
|---|---|
| Molecular Weight(MW) | 76 kDa (note) (3) |
| Cellular Localization | Centrosome |
Application
GCP4 antibody detects GCP4 protein at cytoplasm and centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GCP4 protein stained by GCP4 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
GCP4 antibody detects TUBGCP4 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract 10 % SDS-PAGE GCP4 antibody dilution: 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using GCP4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.| Positive Control | NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
