Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Gemin 3 antibody at 1:250 dilution.| Product Name | Gemin 3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Gemin 3 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 20)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Gemin 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434H052 antibody DP103 antibody GEMIN3 antibody DDX20 antibody DEAD box protein DP 103 antibody "DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20 103kD antibody" probable ATP-dependent RNA helicase DDX20 antibody SMN-interacting protein antibod |
|---|---|
| Molecular Weight(MW) | 92 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , gem |
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Gemin 3 antibody at 1:250 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
