Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000Application
Immunoprecipitation of Geminin protein from HCT-116 whole cell extracts using 5 μg of Geminin antibody . Western blot analysis was performed using Geminin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Geminin antibody at 1:500 dilution.Application
Geminin antibody detects Geminin protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Geminin antibody dilution: 1:250.| Product Name | Geminin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Geminin (geminin)Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. |
| Immunogen | Full length human Geminin Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Gem antibody RP3-369A17.3 antibody GMNN antibody geminin antibody "geminin DNA replication inhibitor antibody" |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) (5) |
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000
Application
Immunoprecipitation of Geminin protein from HCT-116 whole cell extracts using 5 μg of Geminin antibody . Western blot analysis was performed using Geminin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Geminin antibody at 1:500 dilution.
Application
Geminin antibody detects Geminin protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Geminin antibody dilution: 1:250.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
