Rabbit Polyclonal antibody to GFAPGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Immunogen
Peptide sequence around aa. 423~427 (E-S-K-Q-E) derived from Human GFAP.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ICC/IFWB
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
FLJ45472 GFAP glial fibrillary acidic protein
Target
GFAP
Molecular Weight(MW)
50 (note)
Application
Application
Immunofluorescence staining of paraffin-embedded Glioma cells of Spinal Cord using GFAP Antibody
Application
Western blot analysis of extract from Rat brain, Rat hippocampus tissue and C6 cells using GFAP Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.