Application
GFM2 antibody detects GFM2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: GFM2 protein stained by GFM2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
GFM2 antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody diluted at a dilution of 1:500.Application
GFM2 antibody detects GFM2 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GFM2 antibody dilution: 1:1000.| Product Name | GFM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GFM2 (G elongation factor, mitochondrial 2)Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in at least three transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GFM2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (84%), Rhesus Monkey (97%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | G elongation factor mitochondrial 2 EF-G2mt EFG2 RRF2mt MRRF2 RRF2 mEF-G 2 hEFG2 MST027 |
|---|---|
| Target | GFM2 |
| Molecular Weight(MW) | 87 kDa (note) (5) |
| Cellular Localization | Mitochondrion |
Application
GFM2 antibody detects GFM2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: GFM2 protein stained by GFM2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
GFM2 antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody diluted at a dilution of 1:500.
Application
GFM2 antibody detects GFM2 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GFM2 antibody dilution: 1:1000.| Positive Control | 293T , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
