Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody [N3C2], Internal diluted at 1:500.Application
GFM2 antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody diluted at 1:2000.| Product Name | GFM2 antibody , Internal |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GFM2 (G elongation factor, mitochondrial 2)Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in at least three transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GFM2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (87%), Rhesus Monkey (98%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EF-G2mt antibody EFG2 antibody MRRF2 antibody MST027 antibody RRF2 antibody RRF2mt antibody hEFG2 antibody mEF-G 2 antibody GFM2 antibody "elongation factor G 2 mitochondrial antibody" "ribosome-releasing factor 2 mitochondrial antibody" MSTP0 |
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| Molecular Weight(MW) | 87 kDa (note) (3) |
| Cellular Localization | Mitochondrion |
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody [N3C2], Internal diluted at 1:500.
Application
GFM2 antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody diluted at 1:2000.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
