Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of methanol-fixed HepG2, using GGA2 antibody at 1:200 dilution.| Product Name | GGA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GGA2 (golgi-associated, gamma adaptin ear containing, ARF binding protein 2)This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GGA2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (92%), Rat (91%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20966 antibody KIAA1080 antibody VEAR antibody GGA2 antibody VHS domain and ear domain of gamma-adaptin antibody "golgi associated gamma adaptin ear containing ARF binding protein 2 antibody" gamma-adaptin-related protein 2 antibody ADP-ribosyl |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of methanol-fixed HepG2, using GGA2 antibody at 1:200 dilution.| Positive Control | HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
