Application
Western blot analysis of Glucocorticoid Receptor (pS211) expression in A549 (A) whole cell lysates.Application
Immunohistochemical analysis of Glucocorticoid Receptor (pS211) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Glucocorticoid receptor (phospho Ser211) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glucocorticoid receptor (phospho Ser211)This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Glucocorticoid Receptor (pS211). The exact sequence is proprietary. |
| Modification | p-Ser211 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GCR GRL GR NR3C1 nuclear receptor subfamily 3 group C member 1 (glucocorticoid receptor) GCCR GCRST |
|---|---|
| Target | Glucocorticoid receptor |
| Tissue Specificity | Recognizes endogenous levels of Glucocorticoid Receptor (pS211) protein. |
Application
Western blot analysis of Glucocorticoid Receptor (pS211) expression in A549 (A) whole cell lysates.
Application
Immunohistochemical analysis of Glucocorticoid Receptor (pS211) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
