Application
Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-GR (Ser226) antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Glucocorticoid Receptor (phospho Ser226) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glucocorticoid Receptor (phospho Ser226)This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011] |
| Immunogen | Peptide sequence around phosphorylation site of serine 226 (L-L-S(p)-P-L) derived from Human GR. |
| Modification | p-Ser226 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nuclear receptor subfamily 3 group C member 1 (glucocorticoid receptor) GRL GCCR GCRST GCR GR NR3C1 |
|---|---|
| Target | Glucocorticoid Receptor (phospho Ser226) |
| Tissue Specificity | The antibody detects endogenous level of GR only when phosphorylated at serine 226. |
Application
Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-GR (Ser226) antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
