Application
Glucocorticoid receptor antibody immunoprecipitates Glucocorticoid receptor protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid receptor protein by 2.5 μg of Glucocorticoid receptor antibody 7.5% SDS-PAGE The immunoprecipitated Glucocorticoid receptor protein was detected by Glucocorticoid receptor antibody diluted at 1:1000.Application
Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
Sample (30 ug of whole cell lysate) A: Jurkat B : Raji 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Glucocorticoid receptor antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Glucocorticoid receptor antibody at 1:250 dilution.| Product Name | Glucocorticoid receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glucocorticoid receptor (nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor))The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glucocorticoid receptor. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cat, Pig, Rabbit, Sheep, Bovine, Guinea pigMouse (89%), Rat (87%), Dog (89%), Cat (93%), Pig (85%), Rabbit (87%), Sheep (90%), Bovine (91%), Guinea pig (85%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GCCR antibody GCR antibody GR antibody GRL antibody NR3C1 antibody nuclear receptor subfamily 3 group C member 1 variant NS-1 antibody nuclear receptor subfamily 3 group C member 1 variant DL-1 antibody glucocorticoid receptor antibody gluc |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) (13) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Glucocorticoid receptor antibody immunoprecipitates Glucocorticoid receptor protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid receptor protein by 2.5 μg of Glucocorticoid receptor antibody 7.5% SDS-PAGE The immunoprecipitated Glucocorticoid receptor protein was detected by Glucocorticoid receptor antibody diluted at 1:1000.
Application
Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
Sample (30 ug of whole cell lysate) A: Jurkat B : Raji 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Glucocorticoid receptor antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Glucocorticoid receptor antibody at 1:250 dilution.| Positive Control | Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
