Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody . The immunolabeling is completely eliminated by treatment with λ-Ptase.| Product Name | GluR1 (phospho Ser845) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GluR1 (phospho Ser845)Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH |
| Modification | p-Ser845 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GLURA HBGR1 GRIA1 GLUH1 GLUR1 GluA1 MGC133252 GluR1-Subunit (phospho S845) GluR1-Subunit (phospho Ser845) GluR1-Subunit (pS845) GluR1-Subunit (pSer845) GluR1-Subunit phospho S845 GluR1-Subunit phospho Ser845 Phospho GluR1-Subunit (S845) Phosp |
|---|---|
| Target | GluR1 |
| Molecular Weight(MW) | 100 (note) |
| Cellular Localization | Interaction with CACNG2 promotes cell surface expression. |
Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody . The immunolabeling is completely eliminated by treatment with λ-Ptase.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
