Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.Application
Glutamate Dehydrogenase antibody detects Glutamate Dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Glutamate Dehydrogenase antibody diluted at 1:750.Application
Glutamate Dehydrogenase antibody detects Glutamate Dehydrogenase protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Glutamate Dehydrogenase antibody at 1:250 dilution.| Product Name | Glutamate Dehydrogenase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glutamate Dehydrogenase (glutamate dehydrogenase 1)L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Sheep, BovineXenopus laevis (91%), Pig (99%), Sheep (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GDH antibody GDH1 antibody GLUD antibody MGC132003 antibody GLUD1 antibody "glutamate dehydrogenase 1 mitochondrial antibody" glutamate dehydrogenase (NAD(P)+) antibody GDH 1 antibody glutamate dehydrogenase 1 antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.
Application
Glutamate Dehydrogenase antibody detects Glutamate Dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Glutamate Dehydrogenase antibody diluted at 1:750.
Application
Glutamate Dehydrogenase antibody detects Glutamate Dehydrogenase protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Glutamate Dehydrogenase antibody at 1:250 dilution.| Positive Control | A549 , HepG2 , HCT116 , *THP1 , *Raw264.7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
