Application
Glutaminase C antibody detects Glutaminase C protein at mitochondria by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody diluted at 1:500. Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of Glutaminase C antibody or Glutaminase C antibody . Western blot analysis was performed using Glutaminase C antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Glutaminase C antibody diluted at 1:500.Application
Glutaminase antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase antibody diluted by 1:5000.| Product Name | Glutaminase C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glutaminase C (glutaminase isoform 3)Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GLS1 GLS GAM glutaminase AAD20 GAC |
|---|---|
| Target | Glutaminase C |
| Molecular Weight(MW) | 65 kDa (note) |
| Tissue Specificity | Specific for GAC but not KGA isoform |
| Cellular Localization | Mitochondrion |
Application
Glutaminase C antibody detects Glutaminase C protein at mitochondria by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody diluted at 1:500. Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of Glutaminase C antibody or Glutaminase C antibody . Western blot analysis was performed using Glutaminase C antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Glutaminase C antibody diluted at 1:500.
Application
Glutaminase antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase antibody diluted by 1:5000.| Positive Control | MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
