Application
Immunoprecipitation of Glutaminase C (GAC) protein from MCF-7 whole cell extracts using 5 μg of Glutaminase C (GAC) antibody . Western blot analysis was performed using Glutaminase C (GAC) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GAC shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody at a dilution of 1:5000.| Product Name | Glutaminase isoform 2 specific antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glutaminase isoform 2 specific (glutaminase)Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GAC glutaminase GLS1 GLS KGA AAD20 GAM |
|---|---|
| Target | Glutaminase isoform 2 specific |
| Molecular Weight(MW) | 73 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunoprecipitation of Glutaminase C (GAC) protein from MCF-7 whole cell extracts using 5 μg of Glutaminase C (GAC) antibody . Western blot analysis was performed using Glutaminase C (GAC) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GAC shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody at a dilution of 1:5000.| Positive Control | MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
