Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 μg of Glycine dehydrogenase antibody [N3C2-2], Internal . Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:400. Blue: Hoechst 33342 staining.| Product Name | Glycine dehydrogenase antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glycine dehydrogenase (glycine dehydrogenase (decarboxylating))The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes; see MIM 238310, MIM 238330, and MIM 238331.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glycine dehydrogenase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, BovineXenopus laevis (86%), Chicken (85%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GCE antibody GCSP antibody HYGN1 antibody MGC138198 antibody MGC138200 antibody GLDC antibody "glycine dehydrogenase [decarboxylating] mitochondrial antibody" glycine decarboxylase P-protein antibody glycine cleavage system P protein antibody gly |
|---|---|
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 μg of Glycine dehydrogenase antibody [N3C2-2], Internal . Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.
Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.
Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.
Application
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:400. Blue: Hoechst 33342 staining.| Positive Control | HePG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
