Application
Sample (30 µg of whole cell lysate) A: HeLa B: NT2D1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using GNAO1 antibody at 1:200 dilution.Application
Sample (5 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GNAO1 antibody dilution: 1:500.Application
Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 μg of GNAO1 antibody . Western blot analysis was performed using GNAO1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | GNAO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GNAO1 (guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GNAO1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (98%), Xenopus laevis (98%), Chicken (98%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686O0962 antibody G-ALPHA-o antibody GNAO antibody GNAO1 antibody "guanine nucleotide binding protein alpha activating polypeptide O antibody" guanine nucleotide-binding protein G(o) subunit alpha antibody "guanine nucleotide binding protein (G |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (3) |
Application
Sample (30 µg of whole cell lysate) A: HeLa B: NT2D1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using GNAO1 antibody at 1:200 dilution.
Application
Sample (5 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GNAO1 antibody dilution: 1:500.
Application
Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 μg of GNAO1 antibody . Western blot analysis was performed using GNAO1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | A549 , HeLa , Jurkat , NT2D1 , 5 ug mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
