Application
GNAT1 antibody [N1C3] detects GNAT1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GNAT1 protein stained by GNAT1 antibody [N1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:3000Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [N1C3] diluted at 1:3000.| Product Name | GNAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GNAT1 (guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1)Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GNAT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Dog, Chicken, Bovine, Guinea pigRat (98%), Zebrafish (92%), Xenopus laevis (92%), Dog (99%), Chicken (96%), Bovine (98%), Guinea pig (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSNBAD3 antibody GBT1 antibody GNATR antibody GNAT1 antibody "transducin rod-specific antibody" "guanine nucleotide-binding protein G(T) alpha-1 subunit antibody" guanine nucleotide-binding protein G(t) subunit alpha-1 antibody rod-type transducin |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
Application
GNAT1 antibody [N1C3] detects GNAT1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GNAT1 protein stained by GNAT1 antibody [N1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:3000
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [N1C3] diluted at 1:3000.| Positive Control | NT2D1 , IMR32 , U87-MG , mouse eye |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
