Application
GNAT2 antibody [N3C3] detects GNAT2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GNAT2 protein stained by GNAT2 antibody [N3C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
GNAT2 antibody detects GNAT2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT2 antibody diluted by 1:5000.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GNAT2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:5000| Product Name | GNAT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GNAT2 (guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2)Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in cones. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GNAT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, BovineMouse (99%), Rat (99%), Zebrafish (87%), Xenopus laevis (91%), Chicken (94%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACHM4 antibody GNATC antibody GNAT2 antibody cone-type transducin alpha subunit antibody guanine nucleotide-binding protein G(t) subunit alpha-2 antibody "transducin cone-specific alpha polypeptide antibody" transducin alpha-2 chain antibody "guan |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
Application
GNAT2 antibody [N3C3] detects GNAT2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GNAT2 protein stained by GNAT2 antibody [N3C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
GNAT2 antibody detects GNAT2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT2 antibody diluted by 1:5000.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GNAT2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:5000| Positive Control | A549 , 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
