Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 protein stained by GNB1 antibody diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000. Blue: Hoechst 33342 staining.Application
Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 μg of GNB1 antibody . Western blot analysis was performed using GNB1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | GNB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GNB1 (guanine nucleotide binding protein (G protein), beta polypeptide 1)Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Chicken, Rhesus Monkey, Bovine, Guinea pigRat (100%), Xenopus laevis (96%), Dog (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GNB1 antibody "beta subunit signal-transducing proteins GS/GI antibody" "G protein beta-1 subunit antibody" guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1 antibody transducin beta chain 1 antibody guanine nucleotide-binding protein |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (4) |
Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 protein stained by GNB1 antibody diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000. Blue: Hoechst 33342 staining.
Application
Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 μg of GNB1 antibody . Western blot analysis was performed using GNB1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , Jurkat , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
