Rabbit Polyclonal antibody to GNG5G proteins are trimeric (alpha-beta-gamma) membrane-associated proteins that regulate flow of information from cell surface receptors to a variety of internal metabolic effectors. Interaction of a G protein with its activated receptor promotes exchange of GTP for GDP that is bound to the alpha subunit. The alpha-GTP complex dissociates from the beta-gamma heterodimer so that the subunits, in turn, may interact with and regulate effector molecules (Gilman, 1987 [PubMed 3113327]; summary by Ahmad et al., 1995) [PubMed 7606925].[supplied by OMIM, Nov 2010]
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GNG5. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouseDogRatBovine
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
guanine nucleotide binding protein (G protein) gamma 5
Target
GNG5
Tissue Specificity
Recognizes endogenous levels of GNG5 protein.
Application
Application
Western blot analysis of GNG5 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Application Notes
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.