Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GOLGA3 antibody at 1:200 dilution.Application
Sample(30 μg of whole cell lysate) A:A431 B:H1299 5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human gastric tissue, using GOLGA3 antibody at 1:100 dilution.| Product Name | GOLGA3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GOLGA3 (golgin A3)The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins which are localized to the Golgi. Its encoded protein has been postulated to play a role in nuclear transport and Golgi apparatus localization. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GOLGA3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GCP170 antibody MEA-2 antibody GOLGA3 antibody Golgin subfamily A member 3 antibody golgi complex-associated protein of 170 kDa antibody golgin-160 antibody golgin-165 antibody "golgi autoantigen golgin subfamily a 3 antibody" Golgi membrane asso |
|---|---|
| Molecular Weight(MW) | 167 kDa (note) |
| Cellular Localization | Cytoplasm , Golgi apparatus , Golgi stack membrane; Peripheral membrane protein |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GOLGA3 antibody at 1:200 dilution.
Application
Sample(30 μg of whole cell lysate) A:A431 B:H1299 5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human gastric tissue, using GOLGA3 antibody at 1:100 dilution.| Positive Control | 293T , A431 , H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
