Application
Immunohistochemical analysis of paraffin-embedded human smooth muscle, using GOLGA5 antibody at 1:100 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLGA5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000| Product Name | GOLGA5 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GOLGA5 (golgin A5)The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (88%), Rhesus Monkey (98%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GOLIM5 antibody RFG5 antibody ret-II antibody GOLGA5 antibody cell proliferation-inducing gene 31 protein antibody "golgi autoantigen golgin subfamily a 5 antibody" golgi integral membrane protein 5 antibody Golgin subfamily A member 5 antibody g |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type IV membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded human smooth muscle, using GOLGA5 antibody at 1:100 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLGA5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
