Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GOLPH2 antibody at 1:500 dilution.Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (20 µg of whole cell lysate) A: HeLa membrane 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLPH2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | GOLPH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GOLPH2 (golgi membrane protein 1)The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GOLPH2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C9orf155 antibody FLJ22634 antibody FLJ23608 antibody GOLPH2 antibody GP73 antibody PSEC0257 antibody bA379P1.3 antibody GOLM1 antibody golgi phosphoprotein 2 antibody golgi membrane protein GP73 antibody Golgi membrane protein 1 antibody "golgi |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (3) |
| Cellular Localization | Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GOLPH2 antibody at 1:500 dilution.
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (20 µg of whole cell lysate) A: HeLa membrane 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLPH2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | HeLa , HeLa membrane |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
