Application
GOLPH2 antibody detects GOLPH2 protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa membrane fraction lysate/extract 10 % SDS-PAGE GOLPH2 antibody dilution: 1:500Application
Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2 antibody at 1:200 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2 antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.Application
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GOLPH2 antibody [C3], C-term dilution: 1:500.Application
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term diluted at 1:5000. Red: Phalloidin, a F-actin marker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | GOLPH2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GOLPH2 (golgi membrane protein 1)The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GOLPH2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C9orf155 antibody FLJ22634 antibody FLJ23608 antibody GOLPH2 antibody GP73 antibody PSEC0257 antibody bA379P1.3 antibody GOLM1 antibody golgi phosphoprotein 2 antibody golgi membrane protein GP73 antibody Golgi membrane protein 1 antibody "golgi |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (2) |
| Cellular Localization | Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein |
Application
GOLPH2 antibody detects GOLPH2 protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa membrane fraction lysate/extract 10 % SDS-PAGE GOLPH2 antibody dilution: 1:500
Application
Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2 antibody at 1:200 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2 antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Application
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GOLPH2 antibody [C3], C-term dilution: 1:500.
Application
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term diluted at 1:5000. Red: Phalloidin, a F-actin marker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa , HeLa membrane fraction |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
