Application
Western blot analysis of GOLPH3 antibody (N-term) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of hela cells using GOLPH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | GOLPH3 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to GOLPH3 (N-term)The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 6~35 amino acids from the N-terminal region of human GOLPH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Golgi phosphoprotein3 Golgi phosphoprotein 3 GPP34 GOLPH3 Coat protein GPP34 |
|---|---|
| Target | GOLPH3 |
| Molecular Weight(MW) | 33.811 kDa (note) |
Application
Western blot analysis of GOLPH3 antibody (N-term) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of hela cells using GOLPH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
