Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000| Product Name | Grancalcin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Grancalcin (grancalcin, EF-hand calcium binding protein)This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Grancalcin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus MonkeyMouse (88%), Rat (87%), Xenopus laevis (81%), Rhesus Monkey (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GCL antibody, GCA antibody, grancalcin antibody, "grancalcin, penta-EF-hand protein antibody", "grancalcin, EF-hand calcium-binding protein antibody", "grancalcin, EF-hand calcium binding protein antibody" |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) (8) |
| Cellular Localization | Cytoplasm , Membrane |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
