Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Grp78 antibody detects Grp78 protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 7.5% SDS-PAGE Grp78 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody diluted at 1:250.Application
Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 antibody diluted at 1:250.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Grp78 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Grp78 (78 kDa glucose-regulated protein)When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Japanese medaka, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (95%), Japanese medaka (95%), Xenopus laevis (99%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BIP antibody FLJ26106 antibody GRP78 antibody MIF2 antibody HSPA5 antibody immunoglobulin heavy chain-binding protein antibody 78 kDa glucose-regulated protein antibody endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody "heat shock |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum lumen , Melanosome |
Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Grp78 antibody detects Grp78 protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 7.5% SDS-PAGE Grp78 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody diluted at 1:250.
Application
Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 antibody diluted at 1:250.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
