Application
Human brain tissue stained withApplication
Human Brain stained with anti-GSK-3 antibody| Product Name | GSK3 alpha & beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to GSK3 ( alpha + beta )GSK3 (Glycogen Synthase Kinase-3 differs from most serine/threonine kinases) in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3a and GSK-3?. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3. |
| Immunogen | Synthetic peptide (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| IHC-P: Use at a dilution of 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min. WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GSK3A 2931 DKFZp686D0638 605004 606784 GSK3 alpha & beta P49840 GSK3B 2932 P49841 |
|---|---|
| Target | GSK3 alpha & beta |
Application
Human brain tissue stained with
Application
Human Brain stained with anti-GSK-3 antibody| Positive Control | Jurkat cells and brain |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min. WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
