Application
Western Blot: Glycogen synthase kinase 3 Phosphospecific Antibody [ - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).| Product Name | GSK3 alpha/beta (phospho Ser9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GSK3 alpha/beta (phospho Ser9)The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser9 of GSK3beta. Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Modification | p-Ser9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot 1:1000: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GSK3 S9 phospho GSK3 phospho S9 GSK3A GSK3 (pS9) phospho GSK3 Ser9 phospho GSK3 GSK3 Ser9 phospho GSK3 phospho GSK3 phospho Ser9 GSK3 (Phospho Ser9) phospho S9 GSK3 phospho Ser9 GSK3 GSK3 (Phospho S9) GSK3 (pSer9) |
|---|---|
| Target | GSK3 (phospho Ser9) |
| Tissue Specificity | Specific for the ~46k GSK3 bata protein phosphorylated at Ser9. Also weakly labels the ~51k GSK3a band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. |
Application
Western Blot: Glycogen synthase kinase 3 Phosphospecific Antibody [ - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).| Application Notes | Western Blot 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | 10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA |
